Assessing genotoxic potential of petroleum refinery wastewater using biomarkers of laboratory exposed and field captured fishes

Petroleum refinery wastes contain mixtures of toxic compounds including Polycyclic Aromatic Hydrocarbons (PAH) which may pose genotoxic threats to the biota. The objective present study was assess potential wastewaters petroleum at Sapugaskanda, Sri Lanka using erythrocytic micronuclei and nuclear abnormalities as biomarkers in fish Oreochromis niloticus exposed wastewater under controlled laboratory conditions field captured fishes from canals close refinery. Erythrocytic peripheral blood were evaluated cytogenetic tests. Patterns fluorescent aromatic bile also examined fixed fluorescence spectrometry PAH exposure. micronuclei, buds notched nuclei highly induced (P < 0.05) O. for 7 days comparison control fish. field-captured fishes, Trichogaster pectoralis Dawkinsia singhala inhabiting water near demonstrated significant induction <0.05) other abnormalities. exposure indicative patterns (naphthalene, phenanthrene, pyrene, benzo(a)pyrene types) detected fishes. results revealed that contains chemicals PAHs. Hence, incorporation genotoxicity tests bioanalytical tools regulating discharge final aquatic ecosystems would be prudent consideration sustainable development goals focusing on ecosystem human safety.